Microscope World Blog

Time 2020-10-28 21:06:17

Web Name: Microscope World Blog

WebSite: http://blog.microscopeworld.com

ID:97555

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Contact Microscope World with questions regarding fluorescence microscopy or capturing images and video of fluorescence microscopy.The pco.panda 4.2 camera combines revolutionary sCMOS technology in a compact design. High quantum efficiency (up to 80%) and low readout noise make this camera suitable for countless applications. The USB 3.1 interface enables ultra-speed data transfer and direct power via the USB cable, making external power supplies redundant. Frame rate = 40 fps @ full resolution 2048 x 2048, 80 fps @ 2048 x1024.The pco.edge 3.1 camera has a sCMOS sensor and is designed for users who require high resolution and high frame rates. This USB3 camera is available in color or monochrome. 50 fps @ full resolution 2048 x 1536, 75 fps @ 1280 x 1024, 160 fps @ 640 x 480.The pco.edge 4.2 camera has sCMOS technology and can be optionally upgraded with a water cooling system. This camera has high quantum efficiency at up to 82% at peak. 100 fps @ RS fast scan 2048 x 2048, 189 fps @ fast scan 1920 x 1080, 420 fps @ fast scan 640 x 480 when using camera link.The pco.edge 4.2 LT camera has sCMOS technology and USB3 output. High quantum efficiency of up to 82% at peak. 40 fps @ full resolution 2048 x 2048, 80 fps @ 1280 x 1024, and 170 fps @ 640 x 480.The pco.edge 5.5 camera has sCMOS technology and can be optionally upgraded with a water cooling system. Quantum efficiency of 60% @ peak. With camera link, the frame rates are high: 100 fps @ RS/GR fast scan 2560 x 2160, 201 fps @ fast scan 1920 x 1080, 450 fps @ fast scan 640 x 480.Contact Microscope World for more information on pco.cameras or a quote.Fluorescence microscopy is a tool used in biology, material science, and biomedical sciences. The fluorescence microscope can reveal the presence of a single molecule. The basic function of a fluorescence microscope is to irradiate the specimen with a desired and specific band of wavelengths, and then to separate the much weaker emitted fluorescence from the excitation light.The fluorescence microscopy images shown below were captured using the Fein Optic RB30-GFP fluorescence microscope and the Lumenera Infinity 3S-1UR microscope camera.Fluorescence microscopy image captured using a 60x objective lens.There are two key areas on a compound biological microscope with Koehler illumination that need to be adjusted in order to set up the Koehler illumination properly: field diaphragm and aperture diaphragm.The microscope's aperture diaphragm can be found on the condenser of the microscope and it is typically a lever that is adjusted, or a built-in slider on the condenser. This is the RB40 microscope, the arrow points to the aperture diaphragm.Start by adjusting the field diaphragm (on the bottom of the microscope where the light is). Focus on your image and open the field diaphragm all the way up so lots of light shines through.Close the aperture diaphragm (on the condenser) down so you can see the edges of it. Is this dark circle centered in your field of view? If not, use the small centering screws on either side of the condenser to move the circle into the center.Open the aperture diaphragm back up until the image contrast is sharp and the edges of the aperture diaphragm are crisp and clear.This video using the Motic BA410 microscope is a great tutorial to watch if you are uncertain of the steps to take when setting up Koehler illumination on your microscope.You can learn more about Koehler illumination and setup here.Microscope information, images from beneath the microscope and educational science projects.

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