Magnetic beads

Time 2020-09-15 20:03:21

Web Name: Magnetic beads

WebSite: http://www.bioclone.us

ID:47849

Keywords:

Magnetic,beads,c4,c8,c18,pheny,dipheny,wax,sax,wcx,scx,amine,aldehyde,NHS,Carbox

Description:

Biomagnetic separation techniques have attracted a great deal of interest in a wide range of applications in biosciences. The techniques have the advantages for quick, simple and flexible handling of either large or micro liter scale biological samples without the need for laborious repeat of pipetting and centrifuging. BcMag Magnetic Beads are uniform, mono-dispersed, spherical, core shell superparamagnetic beads which consist of a nanometer-scale superparamagnetic iron oxide core completely encapsulated by a high purity silica shell. Silica provides an excellent chromatography separation medium for purification of target molecules thanks to its superior mechanical and chemical stability. Applications: Affinity Beads: Can be used to conjugate almost all function groups Reversed-Phase Beads: C4, C8, C18 Ion-Exchange Beads: WAX, SAX, WCX, SCX Immunoprecipitation Antibody Purification: Protein A, Protein G, Protein A/G, Protein L DNA/RNA Conjugation Purification: Genomic DNA, cDNA, RNA Quick Heteroduplex DNA Finder Biotin-related: Streptavidin, monoavidin Recombinant Protein Purification Endotoxin Removal. Protemics; HSA, IgG .Removal EDTA, IDA BcMag Reversed-Phase magnetic beads are uniform, silica-based superparamagnetic beads coated with high density hydrophobic groups on the surface. The beads are specifically designed, tested and quality controlled for quickly purifying, desalting and concentrating femtomolar to picomolar scale of peptides or proteins from biological fluids and other aqueous samples, manually or automatically without the need for laborious repeat of pipetting and centrifuging. Figure 1 is a Typical MALDI-TOF MS spectra (1-10 kDa) of LMW serum fraction processed on 1um C18 magnetic beads. (Courtesy of Dr.Radoslav (Rado) Goldman, Georgetown University Medical Center ) Features and Advantages:Eluted by mild elution (2mM biotin)High throughput procedure; eliminates columns or filters, or laborious repeat of pipetting or centrifugation (Fig.1)High binding capacity and elute bound biotinylated molecules in mild conditionPurifies biotinylated products under mild elution conditions Exhibits little nonspecific binding Features and Advantages:Quick, easy and one-step high-throughput procedure (Fig.1)Protein/DNA recovery can reach ~95%Broader working pH range (pH4 -10)No sample dilutionScalable - easily adjusts for sample size and automation ( l ~Liter)High binding capacity: 10,000 E.U./mlBeads can be reused at least 5 times. Features and Advantages:Quick, Easy and one-step high-throughput procedure; eliminates columns or filters, or laborious repeat of pipetting or centrifugation (Fig.1)Identify DNA mutations,deletion, and insertion in high efficient, one-step procedureScalable - easily adjusts for sample size and automationReproducible results EcoMag Quick Peptide Conjugation Kit is specifically designed for quick and efficient conjugation of peptides to Long-arm Iodoaetyl-activated magnetic particles for antibody purification from serum of various animal species .Features and AdvantagesPeptide Conjugation:Covalently and specifically couples with cysteine residuesWater-insoluble ligands can be conjugated in coupling buffer containing final concentration of 10-30% acetonitrile, or dimethylformamide (DMF), or dimethylsulfoxide (DMSO), or of 6 M Guanidine HCl.Stable covalent bond with minimal ligand leakageProduces reusable immunoaffinity matricesLow nonspecific bindingImmobilize 1-1.5 mg peptide/ml beadsAntibody Purification:15-20 minutes protocolQuick, easy-to-use, and one-step procedure; eliminates columns or filters, or laborious repeat of pipetting or centrifugation,Low nonspecific binding: Purity ~ 90%Suitable for antibody purification from various animal species No pre-filtration of sample High degree of purity: ~ 90%High biding capacity: 2mg antibody/mlLow cost DNA PurificationFeatures and Advantages:Quick, Easy and one-step high-throughput procedure;eliminates columns or filters,or laborious repeat ofpipetting or centrifugation No need for toxic phenol/chloroform. No need for ethanol in Wash buffer to avoid possible residual ethanol in Elution buffer.Ultra-pure DNA ready for PCR, restriction digestion and other downstream DNA applicationsRNA PurificationFeatures and Advantages Quick, Easy and one-step high-throughput procedure; eliminates columns or filters, or laborious repeat of pipetting or centrifugation No need for toxic phenol/chloroformUltra-pure RNA ready for all downstream RNA applicationsLow costPrice: Call for quotation100% Accuracy Codon Optimization: FreeCloning into Any Vector: FreeTurnaround Time: 15 Days Just send us your DNA template or amino acid sequence , we will return you ultrapure protein with low cost Just send us you samples, we will conjugate them to our magnetic beads with low cost EcoMag His-co2+/Ni2+ Magnetic Beads40% Off 15ml/only $385 Near Neutral pH ElutionPurity: 90%High Binding Capacity: ~5mg/ml of beadsLow cost: 100 ml/only $1610 Long-arm Iodoacetyl-activated Magentic Particles40% off 1,000 ml/only $12,000 Codon-optimized cDNA and Recombinant Protein Hundreds of thousands of Recombinant Proteins andCodon-optimized cDNA Clones Are AvailableThe number of recombinant proteins used for academic research and therapeutic applications has increased dramatically. However, successful recombinant protein expression depends on so many factors such as codon preference, RNA secondary structure, GC content in heterologous expression systems. More and more experimental results proved that the expression level was dramatically increased, from two to one hundred times depending on different genes, when compared with pre-optimization. Bioclone has developed a unique cDNA (cloned in E.coli expression Vector, Fig.1) and recombinant protein (Produced in E.coli Yeast). Bioclone provides ready-to-use and customer-based service for all the cDNA clones and recombinant proteins.Those cDNA clones and recombinant proteins include:Infection disease antigens (Virus, Bacterial, Parasites), Nuclear Protein, Cytokines, Kinase, G-protein, Receptor,Phosphatase, Protease, Signal Transduction, Apoptosis, Stem Cell and Development, Extracellular Matrix, Neuroscience, Toxicology, Drug metabollism, Common Disease, Transcription factors and Cancer.Read More For cDNA or Recombinant Protein Search ( Input Gene name, or Protein Name, or Swiss -prot Accession Number

TAGS:Magnetic beads c4 c8 c18 pheny dipheny wax sax wcx scx amine aldehyde NHS Carbox

<<< Thank you for your visit >>>

Magnetic beads Magnetic particle magnetic purification magnetic bead affinity ion-exchange reversed-phase magnetic beads for DNA RNA protein peptide antibody bacteria and virus parasite condon-optimized cDNA recombinant protein

Hot Websites