Purdue University Cytometry Laboratories | Welcome, PUCL is the leading cytometry site since 1993.

Time 2020-07-29 13:08:29

Web Name: Purdue University Cytometry Laboratories | Welcome, PUCL is the leading cytometry site since 1993.

WebSite: http://cyto.purdue.edu

ID:38607

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Laboratories,Welcome,PUCL,

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The Purdue group has a new DVD-Booklet package focused on Global Health, aimed at sharing ideas and information about innovative programs in resource-limited countries. We will freely distribute 100,000 copies around the world. Publication date for this product was May 2009. ..This is a high profile project and we encourage individuals and institutions to distribute copies. To do so, please send an email to: disc11@flowcyt.cyto.purdue.edu or go to Global Health DVDA significant amount of sponsorship is available for this project. With 20,000 distribution, this project will be very attractive to sponsors. To inquire about furture opportunities please contact us at: disc11sponsor@flowcyt.cyto.purdue.edu Dr. J. Paul Robinson, PUCL Director has just returned from Nepal having sucessfully summited the world s tallest mountain at 29,035ft.The effort was closely related to raising awareness of the devastating problems of AIDS in resource-limited countries, with the goal of raising money for Purdue s nonprofit Cytometry for Life program.C4L is developing low-cost diagnostics to help HIV/AIDS victims in rural Africa receive treatment. You can follow the timeline of the climb his blog. J. Paul Robinson LabGoogle Scholar Link to see our publicationsLASER INDUCED BREAKDOWN SPECTROSCOPY:We have combinedLaser Induced Breakdown Spectroscopy(LIBS) withlanthanide-antibody tagsto create a unique way to detect pathogens or toxins. We currently designed and built a custom LIBS instrument and we plan to move this to a field deployable hand-held instrument for biological detection. The opportunities extend from bioterrorism, to food pathogens and chemical contaminants. This exciting technology has the opportunity for an innovative approach that incorporates detection, diagnostics and quantification.ELASTIC LIGHT SCATTER:We developed a technology foridentification of bacterial pathogensusing elastic light scatter (ELS). It uses a laser to interrogate a bacterial colony and the scatter forms a unique pattern onto an imaging sensor. We have developed a number of projects from this technology including new approaches to classification, instrumentation and automation.Here is a link that explanins ELS.... and lots more on this pageSINGLE PHOTON SPECTROSCOPY:We are very excited about the opportunities in expanding our current PMT/APD photon measurement technologies to single photon (SP) detection. Of particular interest is the unique expansionof spectral analysis in flow cytometry using single photon detectors. Here is a link to a recent paper on single photon detector design by our collaborator Masaonobu Yamamoto. We have developed our own unique high-speed, low-noise detector and we are moving toward building a 42charray detector which we believe will be transformational in biological imaging and the first such detector ever created. The opportunities for integration into multiple detection technologies are endless! Single photon detectorswilloneday replace many currentphoton detectors. Here is a link toa recent book we published on this topicNANO-PARTICLE DETECTION:It seems everyone wants to studynano-particles (NP) but few if any technologies can actually achieve measurement of single NPs and let alone characterize them. We have designed a Blu-Ray based technology as aPoint Of Care (POC) technology that can analyze particles as small as 20nm. We want to advance this technology by integrating multiple fluorescent detection options, and in the future determine how to sort or separate these particles. There are many applications for this technology detection of cancer-markers in plasma, monitoring cancer after therapy, monitoring HIV or HBV or potentially any perhaps any viral disease or even plastic particles in the environment.Here is a Youtube link about this projectSome of our Software Projects1. High Throughput Flow Cytometry Analysis New Book from Purdue University Cytometry Laboratories and University of Modena, Italyhttp://link.springer.com/book/10.1007%2F978-981-10-4499-1Chapters1. Microenvironment Cytometry Paul J. Smith, Victoria Griesdoorn, Oscar F. Silvestre, Rachel J. Errington2. Rare Cells: Focus on Detection and Clinical Relevance Sara De Biasi, Lara Gibellini, Milena Nasi, Marcello Pinti, Andrea Cossarizza3. E All ottavo Giorno, Dio Cre La Citometria and on the 8th Day, God Created Cytometry J. Paul Robinson4. Cytomics of Oxidative Stress: Probes and Problems Jos -Enrique O Connor, Guadalupe Herrera, Francisco Sala-de-Oyanguren, Beatriz J vega, and Alicia Mortinex-Romeroa5. Flow Cytometry in Multi-center and Longitudinal Studies Anis Larbi6. Validation The Key to Translatable Cytometry in the 21st Century Virginia Litwin, Cherie Green, Alessandra Vitaliti7. Flow Cytometry in Microbiology: The Reason and the Need Cid lia Pina-Vaz, Sofia Costa-de-Oliveira, Ana Silva-Dias, Ana Pinto Silva, Rita Teixeira-Santos and Ac cio Gon alves Rodrigues8. Flow Cytometer Performance Characterization, Standardization, and Control Lili Wang, Robert A. Hoffman9. Alternative Approaches for Analysis of Complex Data Sets in Flow Cytometry Carmen Gondhalekar10. Photon Detection: Current Status Masanobu Yamamoto11. Identification of Small-Molecule Inducers of FOXP3 in Human T Cells Using High-Throughput Flow Cytometry Rob Jepras, Poonam Shah, Metul Patel, Steve Ludbrook, Gregory Wands, Gary Bonhert, Andrew Lake, Scott Davis and Jonathan Hill12. Cancer Stem Cells and Multi-drug Resistance by Flow Cytometry Jordi Petriz We present cytometry and confocal microscopy education and research material, maintain the cytometry email archive,and links to cytometry web sites and suppliers worldwide.Our goal is to share high-quality information and to maintain electronic crossroad for cytometry. In 1993, we established the first web site in cytometry. It has matured and evolved with time. We did this because of our interest and dedication to the philosophy of sharing educational material.A new project we are involved with is an NIH SPARC grant - you can see this project here

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